雌性安徽白山羊胸腺形态学增龄性变化

doi:10.11843/j.issn.0366-6964.2018.08.020

摘要/Abstract

摘要：

旨在探究雌性安徽白山羊胸腺组织形态学增龄性变化。选用幼龄期、初情期前、初情期及成年期雌性安徽白山羊各3只，记录胸腺常规指标，H.E.染色观察胸腺组织结构；利用免疫组化分析S100和Caspase-3的分布；采用RT-PCR技术检测胸腺中的S100、CASP-3、CASP-8以及TP53 mRNA的转录水平变化。结果表明：1）胸腺长度、重量、指数和最宽值均在初情期前或初情期达到最大。2）除幼龄期山羊胸腺外，初情期、初情期前和成年期胸腺皮髓质界限清晰。3）Caspase-3阳性细胞广泛分布在皮质和髓质，S100主要分布在髓质和皮髓质交界区域。4）S100 mRNA在各时期的转录水平均无显著性差异（P>0.05）；CASP-3和CASP-8 mRNA在初情期前转录水平最高，TP53 mRNA在成年期水平最高。综上表明，雌性山羊胸腺自初情期开始呈现增龄性退化现象。CASP-3和CASP-8可能参与胸腺的发育和细胞更新以及退化早期过程，而CASP-8和TP53对成年后的胸腺退化更具重要作用。

Abstract:

In order to study the age-related histomorphological changes in the thymus of female Anhui white goats at different stages of growth, 3 female Anhuai white goats in 4 growth periods(infancy, prepuberty, puberty, adulthood) were selected, respectively; and the thymus general indexes were recorded. Hematoxylin-eosin (H.E.) staining was used to observe the morphological structure of the thymus; immunohistochemistry was used to explore the distribution of S100 and Caspase-3 in thymus; in addition, the transcription levels of S100, CASP-3, CASP-8 and TP53 mRNA in thymus were detected using real-time fluorescent PCR (RT-PCR). The results were as follows:1) The length, weight, index and maximum width of the thymus reached their peaks in goats at prepuberty or puberty. 2) The boundaries between the cortex and medulla in the thymus were clear in female goats at prepuberty, puberty and adulthood, but not in infancy. 3) Caspase-3 positive cells were concentrated at the cortex and medulla of the thymus, but S100 was mainly distributed in the junction of the cortex and medulla. 4) No significant difference was found in S100 mRNA levels between different periods (P>0.05); CASP-3 and CASP-8 mRNA levels were highest at prepuberty, and TP53 mRNA was the highest at adulthood of female goats. The results indicated that female goats' thymuses showed age-related deterioration after puberty. CASP-3 and CASP-8 may participate in thymus development, cell renewal and involution processes in the early periods of development. Additionally, CASP-8 and TP53 play important role in thymus involution during adulthood.

中图分类号:

S827
S854.4

蒋书东, 张兴旺, 方富贵, 周伟娟, 王登峰, 朱晖. 雌性安徽白山羊胸腺形态学增龄性变化[J]. 畜牧兽医学报, 2018, 49(8): 1743-1751.

JIANG Shu-dong, ZHANG Xing-wang, FANG Fu-gui, ZHOU Wei-juan, WANG Deng-feng, ZHU Hui. Age-related Thymic Morphology Changes in the Female Anhui White Goat[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(8): 1743-1751.

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参考文献

[1] MILLER J F A P.Immunological function of the thymus[J].Lancet,1961,278(7205):748-749.
[2] 苏时萍.GnRH主动免疫对下丘脑-垂体-性腺-胸腺环路的调节机制[D].合肥:安徽农业大学,2013.
SU S P.The regulation of active immunization against GnRH on hypothalamus-pituitary-gonadal thymus loop[D].Hefei:Anhui Agricultural University,2013.
[3] HAYNES B F,SEMPOWSKI G D,WELLS A F,et al.The human thymus during aging[J].Immunol Res,2000,22(2-3):253-261.
[4] 张倩,余四九,何俊峰,等.牦牛胸腺增龄性变化的形态学观察[J].畜牧兽医学报,2013,44(8):1305-1310.
ZHANG Q,YU S J,HE J F,et al.Morphologycal observation of age-associated changes in the thymus of the yak[J].Acta Veterinaria et Zootechnica Sinica,2013,44(8):1305-1310. (in Chinese)
[5] 魏岚,刘丽,徐玉东,等.小鼠胸腺结构的增龄性变化[J].中国老年学杂志,2007,27(9):832-835.
WEI L,LIU L,XU Y D,et al.Ageing changes in thymic structure of mice[J].Chinese Journal of Gerontology,2007,27(9):832-835. (in Chinese)
[6] HALE L P,CLARK A G,LI J,et al.Age-related thymic atrophy in the guinea pig[J].Dev Comp Immunol,2001,25(5-6):509-518.
[7] TORROBA M,ZAPATA A G.Aging of the vertebrate immune system[J].Microsc Res Tech,2003,62(6):477-481.
[8] GRUVER A L,HUDSON L L,SEMPOWSKI G D.Immunosenescence of ageing[J].J Pathol,2007,211(2):144-156.
[9] MACKALL C L,PUNT J A,MORGAN P,et al.Thymic function in young/old chimeras:Substantial thymic T cell regenerative capacity despite irreversible age-associated thymic involution[J].Eur J Immunol,1998,28(6):1886-1893.
[10] 刘仰斌,李启华.小鼠胸腺形态结构的增龄性变化[J].中国组织工程研究与临床康复,2010,14(31):5805-5808.
LIU Y B,LI Q H.Age-related morphology and structure changes of mouse thymus[J].Journal of Clinical Rehabilitative Tissue Engineering Research,2010,14(31):5805-5808. (in Chinese)
[11] ZIMMER D B,CORNWALL E H,LANDAR A,et al.The S100 protein family:History,function,and expression[J].Brain Res Bull,1995,37(4):417-429.
[12] PEARSE G.Normal structure,function and histology of the thymus[J].Toxicol Pathol,2006,34(5):504-514.
[13] STEINMANN G G.Changes in the human thymus during aging[C]//MVLLER-HERMELINK H K. The Human Thymus.Berlin,Heidelberg:Springer,1986:43-88.
[14] SHANLEY D P,AW D,MANLEY N R,et al.An evolutionary perspective on the mechanisms of immunosenescence[J].Trends Immunol,2009,30(7):374-381.
[15] LUBIS I,LADDS P W,REILLY L R,et al.Age associated morphological changes in the lymphoid system of tropical cattle[J].Res Vet Sci,1982,32(3):270-277.
[16] PLECAŠ-SOLAROVIC B,PEŠIC V,RADOJEVIC K,et al.Morphometrical characteristics of age-associated changes in the thymus of old male wistar rats[J].Anat Histol Embryol,2006,35(6):380-386.
[17] SHINZATO M,SHAMOTO M,HOSOKAWA S,et al.Differentiation of Langerhans cells from interdigitating cells using CD1a and S-100 protein antibodies[J].Biotech Histochem,1995,70(3):114-118.
[18] 孙宇,陆江阳,王晓虹,等.小鼠胸腺树突状细胞的分布与形态学观察[J].解放军医学杂志,2004,29(9):813-815.
SUN Y,LU J Y,WANG X H,et al.Observation of the morphology and the distribution of mouse thymus dendritic cells[J].Medical Journal of Chinese People's Liberation Army,2004,29(9):813-815. (in Chinese)
[19] RESENDES A R,MAJÍ N,SEGALÉS J,et al.Apoptosis in normal lymphoid organs from healthy normal,conventional pigs at different ages detected by TUNEL and cleaved caspase-3 immunohistochemistry in paraffin-embedded tissues[J].Vet Immunol Immunopathol,2004,99(3-4):203-213.
[20] 周瑞祥,黄云梅,刘卉,等.胸腺细胞凋亡的死亡信号表达[J].解剖学杂志,2006,29(2):171-173,212.
ZHOU R X,HUANG Y M,LIU H,et al.Expression of death signal pathway in thymocyte apoptosis[J].Chinese Journal of Anatomy,2006,29(2):171-173,212. (in Chinese)